"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."
Janet Duerr, Ohio UniversityRating: 5.0
DyLight™ 405 - conjugated secondary antibodies are excited maximally at about 400 nm and fluoresce with a peak at about 421 nm. They are very bright and photostable, but their optimal use requires instruments equipped with a 405 nm laser and a 420 nm emission filter.
The combination of DyLight 405, Alexa Fluor® 488, Rhodamine Red™-X, and Alexa Fluor® 647 provides for maximum color separation, good photostability and high sensitivity.
DyLight 405 is available conjugated to:
| Excitation Peak (nm) | Emission Peak (nm) | |
|---|---|---|
| DyLight 405 | 400 | 421 |
| Control Reagents | Product Code |
|---|---|
| DyLight™ 405-conjugated ChromPure Donkey IgG, whole molecule | 017-470-003 |
| DyLight™ 405-conjugated ChromPure Goat IgG, whole molecule | 005-470-003 |
| DyLight™ 405-conjugated ChromPure Human IgG, whole molecule | 009-470-003 |
| DyLight™ 405-conjugated ChromPure Mouse IgG, whole molecule | 015-470-003 |
| DyLight™ 405-conjugated ChromPure Rabbit IgG, whole molecule | 011-470-003 |
| DyLight™ 405-conjugated ChromPure Rat IgG, whole molecule | 012-470-003 |
| DyLight™ 405-conjugated ChromPure Sheep IgG, whole molecule | 013-470-003 |
| DyLight™ 405-conjugated ChromPure Bovine IgG, whole molecule | 001-470-003 |
| Streptavidin | Product Code |
| DyLight™ 405-conjugated Streptavidin | 016-470-084 |
Use the spectra viewer below to compare DyLight 405 with other fluorophores, along with instrument specifics (laser/filter) for suitability in your assay.
