"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."
Janet Duerr, Ohio UniversityRating: 5.0
Alexa Fluor® 680 and Alexa Fluor® 790 conjugates are used for very sensitive Western blots, ELISAs, and multiplexing arrays. Alexa Fluor® 680 conjugates are excited with a peak around 684 nm and fluoresce with a peak around 702 nm. Alexa Fluor® 790 conjugates are excited with a peak around 792 nm and fluoresce at a peak around 803 nm. They are the best choice for highly sensitive single or double labeling with fluorescence imaged in a LI-COR Odyssey® imager.
Dye |
Laser line (nm) |
Common filter set |
Excitation (nm) |
Emission (nm) |
Extinction co-efficient |
Quantum Yield (Φ) |
Molecular Weight (kDa) |
Applications |
|---|---|---|---|---|---|---|---|---|
| Alexa Fluor® 680 | 633 | 684 | 702 | 1.4 | N/A | 1150 | Microscopy, Flow Cytometry, Fluorisa, Fluorescent Western Blotting | |
| Alexa Fluor® 790 | 792 | 803 | 1.4 | N/A | 1750 | Microscopy, Flow Cytometry, Fluorisa, Fluorescent Western Blotting |
| Dye | Alexa Fluor® 680 |
|---|---|
| Laser line (nm) | 633 |
| Common filter set | |
| Excitation (nm) | 684 |
| Emission (nm) | 702 |
| Extinction co-efficient | 1.4 |
| Quantum Yield (Φ) | N/A |
| Molecular Weight (kDa) | 1150 |
| Applications | Microscopy |
| Flow Cytometry | |
| Fluorisa | |
| Fluorescent Western Blotting |
| Dye | Alexa Fluor® 790 |
|---|---|
| Laser line (nm) | |
| Common filter set | |
| Excitation (nm) | 792 |
| Emission (nm) | 803 |
| Extinction co-efficient | 1.4 |
| Quantum Yield (Φ) | N/A |
| Molecular Weight (kDa) | 1750 |
| Applications | Microscopy |
| Flow Cytometry | |
| Fluorisa | |
| Fluorescent Western Blotting |
| Control Reagents | Product Code |
|---|---|
| Alexa Fluor® 680-conjugated ChromPure Donkey IgG, whole molecule | 017-620-003 |
| Alexa Fluor® 790-conjugated ChromPure Donkey IgG, whole molecule | 017-650-003 |
| Alexa Fluor® 680-conjugated ChromPure Goat IgG, whole molecule | 005-620-003 |
| Alexa Fluor® 790-conjugated ChromPure Goat IgG, whole molecule | 005-650-003 |
| Alexa Fluor® 680-conjugated ChromPure Mouse IgG, whole molecule | 015-620-003 |
| Alexa Fluor® 790-conjugated ChromPure Mouse IgG, whole molecule | 015-650-003 |
| Streptavidin | Product Code |
| Alexa Fluor® 680-conjugated Streptavidin | 016-620-084 |
| Alexa Fluor® 790-conjugated Streptavidin | 016-650-084 |
Use the spectra viewer below to compare Alexa Fluor® 680 and 790 with other fluorophores, along with instrument specifics (laser/filter) for suitability in your assay.
