"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."
Janet Duerr, Ohio UniversityRating: 5.0
NEW Alexa Fluor® 555 and Alexa Fluor® 568 dyes are conjugated to Donkey and Goat host species secondary antibodies designed with multiple labeling in mind as they are minimally cross-reactive to many common species. Combine Alexa Fluor® 555 and 568 with our full range of JIR Alexa Fluor® conjugated secondary antibodies to design labeling experiments that access the full spectrum!
| Alexa Fluor® | Excitation Peak (nm) | Emission Peak (nm) | |
|---|---|---|---|
| Alexa Fluor® 488 | 493 | 519 | |
| NEW! | Alexa Fluor® 555 | 552 | 572 |
| NEW! | Alexa Fluor® 568 | 577 | 602 |
| Alexa Fluor® 594 | 591 | 614 | |
| Alexa Fluor® 647 | 651 | 667 | |
| Alexa Fluor® 680 | 684 | 702 | |
| Alexa Fluor® 790 | 792 | 803 |
Alexa Fluor® dye conjugates are the industry standard for brightness and usability. They are highly water-soluble and are fluorescent across a wide pH range. They offer superb brightness and photostability with minimal background and are compatible with the most common excitation sources. Jackson ImmunoResearch introduces two new dyes to its portfolio, Alexa Fluor® 555 and 568, offering you two more choices in the orange channel for more flexible panel design!
| Dye | Alexa Fluor® 555 |
|---|---|
| Laser line (nm) | 488/532 |
| Common filter set | 555/20 |
| Excitation (nm) | 552 |
| Emission (nm) | 572 |
| Extinction co-efficient | 1.4 |
| Quantum Yield (Φ) | 0.1 |
| Molecular Weight (kDa) | 1250 |
| Applications | Microscopy |
| Flow Cytometry | |
| Fluorisa | |
| Fluorescent Western Blotting |
| Dye | Laser line (nm) | Excitation (nm) | Emission (nm) | Extinction co-efficient | Quantum Yield (Φ) | Molecular Weight (kDa) | Applications |
|---|---|---|---|---|---|---|---|
| Alexa Fluor® 555 | 488/532 | 552 | 572 | 1.4 | 0.1 | 1250 | Microscopy, Flow Cytometry, Fluorisa, Fluorescent Western Blotting |
Alexa Fluor® 555 conjugated antibodies absorb light maximally around 552 nm and fluoresce with a peak around 572 nm. Alexa Fluor® 555 dye is a bright, orange-fluorescent dye with excitation ideally suited to the 555 nm laser line. Alexa Fluor® 555 dye is pH-insensitive over a wide molar range, providing stable signal generation for flow cytometry and imaging applications, including some super-resolution protocols (Goossen-Schmidt et al., 2020). Dye molecules like Alexa Fluor® 555, with high fluorescence quantum yield and high photostability, allow the detection of low-abundance biological structures with great sensitivity.
| Dye | Alexa Fluor® 568 |
|---|---|
| Laser line (nm) | 568 |
| Common filter set | |
| Excitation (nm) | 577 |
| Emission (nm) | 602 |
| Extinction co-efficient | 1.4 |
| Quantum Yield (Φ) | 0.69 |
| Molecular Weight (kDa) | 792 |
| Applications | Microscopy |
| SRM | |
| Flow Cytometry | |
| Fluorisa | |
| Fluorescent Western Blotting |
| Dye | Laser line (nm) | Excitation (nm) | Emission (nm) | Extinction co-efficient | Quantum Yield (Φ) | Molecular Weight (kDa) | Applications |
|---|---|---|---|---|---|---|---|
| Alexa Fluor® 568 | 568 | 577 | 602 | 1.4 | 0.69 | 792 | Microscopy, SRM, Flow Cytometry, Fluorisa, Fluorescent Western Blotting |
Alexa Fluor® 568 conjugated antibodies absorb light maximally around 577 nm and fluoresce with a peak around 602 nm. Alexa Fluor® 568 dyes are bright, orange-fluorescent dyes with excitation ideally suited for the 568 nm laser line on the Ar-Kr mixed-gas laser. Alexa Fluor® 568 dyes are pH-insensitive over a wide molar range. Alexa Fluor® 568 is a bright and photostable orange-red dye that matches well with Red Fluorescent Protein (RFP) filter sets and may be used in stochastic optical reconstruction microscopy (STORM)—as a reporter in dSTORM (Goossen-Schmidt et al., 2020).
Alexa Fluor® 555 and 568 are available conjugated to:
