"The anti-human polyclonal capture reagents are some of the most robust reagents I have used in my biophysical and biochemical studies so far. Overall, the anti-Fc reagents regardless of the host or capturing species provides a robust capturing ability with minimum cross-reactivity to other species." Full review.
Rupesh Nanjunda,Rating: 5.0
Rapid assays like lateral flow tests provide quick yes or no results in an easy to use format. They allow for point of care analysis as an alternative to more time-consuming immunoassays that need to be performed in laboratories. Rapid tests are used to qualitatively detect patient-generated antibodies to enable clinical diagnosis and indicate treatment.
Lateral flow or rapid tests use the principle of immunochromatography - whereby the components of the test flow laterally through a matrix by capillary action allowing the antigens to combine with antibodies to facilitate the detection and visualization of those complexes.
Step 1. Lateral flow test strips commonly consist of a sample pad, a conjugate pad that has been impregnated with conjugated antibodies, for example, gold conjugated Goat Anti-Human Fcγ specific antibodies and two “lines” which have been applied to nitrocellulose with antigen (T), and control antibodies, for example, Bovine Anti-Goat (C).
Step 2. The sample is introduced to the sample pad via a hole in the test strip cassette.
Step 3. Capillary action draws the sample into the conjugate pad and rehydrates the impregnated antibody conjugates. Any antibodies in the sample to which the conjugated antibody is specific will form complexes.
Step 4. The sample and complexed antibodies migrate onto the antigen-coated nitrocellulose (T) test “line” where they interact.
Step 5. If the antibodies in the sample are specific to the coated antigen the antibody complexes will be deposited and develop color over the line giving a visual indication of a “positive” test. If there are no antibodies specific to the antigen in the test sample, no colored line will develop. Un-complexed gold conjugated Goat Anti-Human antibodies continue to migrate through the test strip to the control line (C).
Step 6. The free conjugated Goat Anti-Human antibodies can be captured by the coated Bovine Anti-Goat IgG and develop a colored line to indicate the test has been performed correctly. No control line would indicate an invalid test.
Step 1. Lateral flow test strips commonly consist of a sample pad, a conjugate pad that has been impregnated with conjugated antibodies, for example, gold conjugated Goat Anti-Human Fcγ specific antibodies and two “lines” which have been applied to nitrocellulose with antigen (T), and control antibodies, for example, Bovine Anti-Goat (C).
Step 2. The sample is introduced to the sample pad via a hole in the test strip cassette.
Step 3. Capillary action draws the sample into the conjugate pad and rehydrates the impregnated antibody conjugates. Any antibodies in the sample to which the conjugated antibody is specific will form complexes.
Step 4. The sample and complexed antibodies migrate onto the antigen-coated nitrocellulose (T) test “line” where they interact.
Step 5. If the antibodies in the sample are specific to the coated antigen the antibody complexes will be deposited and develop color over the line giving a visual indication of a “positive” test. If there are no antibodies specific to the antigen in the test sample, no colored line will develop. Un-complexed gold conjugated Goat Anti-Human antibodies continue to migrate through the test strip to the control line (C).
Step 6. The free conjugated Goat Anti-Human antibodies can be captured by the coated Bovine Anti-Goat IgG and develop a colored line to indicate the test has been performed correctly. No control line would indicate an invalid test.
Jackson ImmunoResearch AffiniPure™ Anti-Human secondary antibodies are affinity purified polyclonal antibodies with specificity for human immunoglobulins.
Read More
ChromPure is our trade name for highly purified proteins from the serum of non-immunized animals.
Read More
